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Coding

Part:BBa_K1189007:Design

Designed by: Taylor Remondini   Group: iGEM13_Calgary   (2013-09-17)

Beta-lactamase with His Tag under the control of the inducible lacI promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design

The bsaI cut site in the gene have been removed. This part has been designed in the RFC 25 Freiburg fusion backbone and can be used to make fusion proteins. The ATG codon is incorporated in the backbone before the NgoMIV restriction cut site.